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May shares traits of both her parents. Like her mother, she is beautiful, charismatic and a popular student. Like her father, she is intelligent and bright. She also inherited his love for in-fight bantering. In addition, she is a very good athlete and excelled in her girls' basketball team until she quit after her powers emerged. On the other hand, May seems to have inherited the "Parker luck" in which her dual identity wreaks havoc in her private life.[citation needed]

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At some point, May went back to calling herself Spider-Girl and attending Midtown High. During a Friday afternoon, she and Davida discussed their plans for a girls' night out, While talking, they were approached by Jason, who offered to let them visit his uncle's new fun center. Davida turned him down due to already having plans, and May revealed that she'd be busy watching Benjy while her parents were out on a date.

Little Sisters (originally named as Gatherers by the game developers) are young girls who have been genetically altered and mentally conditioned to reclaim ADAM from corpses around Rapture. Little Sisters are almost always accompanied by a Big Daddy. They generally are immune to damage, outside of being "harvested", and have no offensive abilities. Attacking them, however, will incur the wrath of their Big Daddy protectors.

ADAM is excreted by unique Sea Slugs found near Rapture. The ADAM-producing Sea Slugs did not naturally produce a large enough quantity for serious research and commercial exploitation. Dr. Brigid Tenenbaum developed a procedure whereby a Sea Slug was implanted in a human host's stomach, and a symbiotic interaction between host and slug yielded up to thirty times the quantity of usable ADAM. Young girls, nicknamed "Little Sisters" following the implantation process, were found to be the only viable hosts. Although Tenenbaum hoped to be able to keep the children in a vegetative state, it was found they had to be fully functional to produce the ADAM. They still maintained many of the ordinary characteristics of small children: laughing, playing, smiling, and singing.[1]

Frank Fontaine created his Little Sister's Orphanage in Apollo Square and other locations as a front to exploit the children as his supply of hosts for ADAM production. When Andrew Ryan took over Fontaine Futuristics, he discovered the existence of the children. He found them appalling, but had to accept the necessity of their existence to generate the quantity of ADAM Rapture now required. It wasn't long before the entire city became aware of where ADAM was really coming from. He shut down those false orphanages, and continued to use the Little Wonders Educational Facility to house the existing ADAM hosts. With the City's ADAM addiction endemic, and ADAM's use to fight the civil war, shortages were becoming critical. Eventually, as the civil war's body counts grew and the shortages increased, a solution to get additional ADAM led to using the children to obtain ADAM from corpses. Little Sister ADAM hosts were mentally and physically prepared for this grim harvesting role. Ryan, to negate the abhorence of the population, portrayed the Little Sisters in a marketing gimmick, their image being used on Plasmid and Tonic vending machines called Gatherer's Gardens, and even produced toy dolls of them. Rapture public announcements introduced them as the salvation of the City, a reassurance of continuing ADAM production.With the growing chaos of the Civil War, the demand for ADAM soared and Ryan Industries resorted to kidnapping little girls from their parents to create more Little Sisters.

Dr. Yi Suchong, realizing that the need for ADAM was greater than the amount produced by the Little Sisters themselves, pioneered a way for them to recycle ADAM from the blood of dead Splicers, greatly increasing their ADAM yield.[2] Naturally, the children were unwilling to cooperate. To overcome this basic obstacle, Dr. Suchong created methods to mentally condition the children. This produced a neurological impulse to recognize the bodies of dead Splicers as "Angels" to make the grisly process of extraction and consuming their ADAM-rich blood appealing for the girls.[3] Like many of the tools in Rapture, the Little Sisters' ADAM extractor syringes were fabricated from common items: large needles, hose nozzles, and baby bottles.

To make Little Sisters more effective in their task, they were heavily conditioned to see the world of Rapture very different from reality. Instead of seeing the dark, deteriorated state of the city, they saw an idealized child's world, with rosy marble floors, pink or red drapings, and flowering vines growing everywhere. Little Sisters saw their Big Daddies as noble golden knights, and saw Big Sisters as brides or princesses with elegant ballroom apparel. Statues appeared to them as heroic Big Daddies, pools of blood as rose petals, flies as butterflies, random fires on the floor as lit fireplaces, weapons as red plastic toy guns, and the dead bodies of Splicers as "Angels"; men and women lying in elegant repose with the outline of a halo and angelic wings surrounding them. Splicers themselves appeared as handsome and beautiful, taking the forms of elegantly dressed men and women in masquerade masks, conversing civilly, when in reality they were violently fighting. This "dream world" persisted in the girls' day-to-day experience, except for occasional lapses where their senses were brought abruptly back to the grim, twisted reality of Rapture's decay whenever they were frightened or startled. This was also shown to happen while concentrating on the process of gathering ADAM from a corpse. However, this could just be due to Delta temporarily losing control over the girl while she harvests ADAM.

With physical and neurological drives in place, the Little Sisters eagerly pursued gathering ADAM in Rapture. The ADAM coursing through their bodies made them virtually indestructible, immediately repairing any damaged tissue. However, they still had the stature and strength of small girls (not to mention their constant hallucinatory state); thus, they were a constant target for abduction and exploitation. Dr. Suchong, in light of the rising mortality rate of Little Sisters, initiated the Protector Program. The idea was to create a series of protectors that would defend the Little Sisters from Splicer attacks so they could gather ADAM without interference. This ambition proved to be more of a challenge than anticipated by the scientists, and numerous attempts were needed to find the right balance of ADAM-based conditioning in both the gatherer and the protector. Despite the tests and trials, through the hands of a third party, the final key to the bond was found to be quid pro quo, as both discover that one could not survive without the other. The results of the program had each Little Sister paired with a Big Daddy, a heavily spliced human test subject grafted into an enormous armored diving suit. After an unfortunate event leading to the demise of Dr. Suchong, the bond was further strengthened through conditioning employing pheromones. The pheromone produced by the Big Daddy attracted the Little Sisters, and its counterpart produced by the Little Sisters instinctively drove the Big Daddies to defend them with their lives. Once Big Daddies came into full production, the Little Sisters were also put through a course of Pavlovian psychological exercises,[4] designed to force them into rejecting all positive feelings towards any mother-type figure, and instead place all of their affection upon a generic Big Daddy archetype. As a result, the little girls see every Big Daddy protecting them as the same person, and consider the mere mention of the word 'Mommy' to be taboo.

After the events of BioShock, Tenenbaum left for the surface, taking many of the saved Little Sisters with her. However, Sofia Lamb soon came into power in Rapture and later restarted the Little Sister program to supply the Rapture Family with ADAM, and to further her own plans. There were not enough young girls left in Rapture to supply the ADAM cycle, and many of those who remained had matured to the point that they were only useful as Big Sisters.

Lamb began a program of kidnapping young girls from the surface to convert them into Little Sisters. Taken by Big Sister protectors, these new Little Sisters were processed in the Paediatric Wing of the Persephone prison colony under the watchful eye of Edward Grimes and his staff of physicians. The new girls developed a unique psychological bond with Eleanor Lamb, who could see through their eyes and even control them to a certain extent.

When Tenenbaum discovered the kidnappings on the surface, she knew immediately where the girls were being taken, and what purpose they were to serve. She acquired a submersible and descended to Rapture expecting to find the missing girls turned into Little Sisters, gathering alongside Big Daddies. So she began rescuing them again. Eventually, Eleanor contacted Tenenbaum through her connection to the Little Sisters, and they created a plan to save the Little Sisters and help Eleanor escape to the surface by resurrecting Subject Delta. After Delta was brought back to life using a Vita-Chamber in the Adonis Luxury Resort, the Little Sisters began to work with him, helping him to reach Eleanor at Persephone. Meanwhile, Tenenbaum reached out to other deactivated Alpha Series Big Daddies throughout Rapture, such as Subject Sigma, and asked for their aid in rescuing the remaining Little Sisters in the city.

Each Little Sister is a young girl between five and ten years of age.[11] In BioShock, the Little Sisters had varying hair colors, but in BioShock 2 they are all brunette, including Cindy Meltzer in game due to time constraints preventing the developers from making her blonde. The girls are clothed in dirty dresses of various colors that reach just below their knees. All of the Little Sisters are barefoot while they explore the halls of Rapture, but before the riots they wore shoes, as evidenced in the opening cutscene of BioShock 2 and Burial at Sea. Due to their fast regeneration, they can walk on any harsh surface without problems. 041b061a72

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